Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae

作者：Azad Gajendra Kumar; Singh Vikash; Thakare Mayur Jankiram; Baranwal Shivani; Tomar Raghuvir Singh^*

来源：BMC Microbiology, 2014, 14(1): 317.

DOI：10.1186/s12866-014-0317-0

• Yeast drug response
• Curcumin
• HOG pathway
• Hog1 phosphorylation
• Glycerol-3-phosphate dehydrogenase 1 (GPD1)
• Mitogen-activated protein kinases

Background: Curcumin (CUR), an active polyphenol derived from the spice turmeric, has been traditionally used for centuries in ancient Indian medicine to treat a number of diseases. The physiological effects of CUR have been shown to be diverse; however, the target molecules and pathways that CUR affects have yet to be fully described. %26lt;br%26gt;Results: Here, we demonstrate for the first time that the budding yeast mitogen-activated protein kinase (MAPK) Hog1 is essential for the response to CUR. Moreover, CUR-induced Hog1 phosphorylation was rescued by supplementation of iron to the growth medium. Hog1 was rapidly phosphorylated upon CUR treatment, but unlike the response to hyperosmotic shock (0.8 M NaCl), it remains activated for an extended period of time. A detailed analysis of HOG pathway mutants revealed that Pbs2p, Ptc2p, and Ssk2p are required for optimal CUR-induced Hog1 phosphorylation. We also observed a Hog1 dependent transcriptional response to CUR treatment that involved the up-regulation of glycerol-3-phosphate dehydrogenase 1 (GPD1), a factor that is essential for the hyperosmotic stress response. %26lt;br%26gt;Conclusions: Our present finding revealed the role of Hog1 MAPK in regulation of CUR-induced transcriptional response. We anticipate that our finding will enhance the understanding on the molecular mode of action of CUR on S. cerevisiae.

• 出版日期2014-12-19