Analysis of Sugar Degradation Products with alpha-Dicarbonyl Structure in Carbonated Soft Drinks by UHPLC-DAD-MS/MS

作者：Gen**erger Sabrina; Glomb Marcus A; Pischetsrieder Monika^*

来源：Journal of Agricultural and Food Chemistry, 2013, 61(43): 10238-10245.

DOI：10.1021/jf3048466

• sugar degradation products
• alpha-dicarbonyl compounds (alpha-DCs)
• carbonated soft drinks (CSD)
• high-fructose corn syrup (HFCS)
• o-phenylenediamine (OPD)

Sugar-sweetened carbonated soft drinks (CSDs) are broadly consumed worldwide. The added sugar, particularly high-fructose corn syrup (HFCS), can be an important source of sugar degradation products, such as alpha-dicarbonyl compounds. This study recorded the alpha-dicarbonyl profile in CSDs by ultrahigh-performance liquid chromatography with hyphenated diode array-tandem mass spectrometry after derivatization with o-phenylenediamine. Thus, 3-deoxy-D-erythro-hexos-2-ulose (3-DG), D-lyxo-hexos-2-ulose (glucosone), 3-deoxy-D-threo-hexos-2-ulose (3-DGal), 1-deoxy-D-erythro-hexos-2,3-diulose (1-DG), 3,4-dideoxyglucosone-3-ene (3,4-DGE), methylglyoxal, and glyoxal were identified as major alpha-dicarbonyls and, with the exception of glyoxal, quantified (recovery rates, 85.6-103.1%; RSD, 0.8-3.6%). Total alpha-dicarbonyl concentration in 25 tested commercial products ranged between 0.3 and 116 mu g/mL and was significantly higher in HFCS-sweetened CSDs compared to CSDs sweetened with HFCS and sucrose or with sucrose alone. Predominant was 3-DG (%26lt;= 87 mu g/mL) followed by glucosone (%26lt;= 21 mu g/mL), 3-DGal (%26lt;= 7.7 mu g/mL), 1-DG (%26lt;= 2.8 mu g/mL), methylglyoxal (%26lt;= 0.62 mu g/mL), and 3,4-DGE (%26lt;= 0.45 mu g/mL).

• 出版日期2013-10-30