Double Transgenic Mice Crossed GFP-LC3 Transgenic Mice With alpha MyHC-mCherry-LC3 Transgenic Mice Are a New and Useful Tool to Examine the Role of Autophagy in the Heart

作者：Terada Mai; Nobori Kiyoshi^*; Munehisa Yoshiko; Kakizaki Manabu; Ohba Takayoshi; Takahashi Yoichiro; Koyama Takashi; Terata Yutaka; Ishida Masaru; Iino Kenji; Kosaka Toshimitsu; Watanabe Hiroyuki; Hasegawa Hitoshl; Ito Hiroshi

来源：Circulation Journal, 2010, 74(1): 203-206.

DOI：10.1253/circj.CJ-09-0589

• Autophagy
• Cardiomyocytes
• Transgenic mouse

Background: The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice.
Methods and Results: We generated transgenic mice expressing mCherry-LC3 under alpha MyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation.
Conclusions: This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74: 203-206)

• 出版日期2010-1