Age-related changes in the gut microbiota influence systemic inflammation and stroke outcome

作者：Spychala, Monica S.; Venna, Venugopal Reddy; Jandzinski, Michal; Doran, Sarah J.; Durgan, David J.; Ganesh, Bhanu Priya; Ajami, Nadim J.; Putluri, Nagireddy; Graf, Joerg; Bryan, Robert M.; McCullough, Louise D.^*

来源：Annals of Neurology, 2018, 84(1): 23-36.

DOI：10.1002/ana.25250

• CENTRAL-NERVOUS-SYSTEM
• CHAIN FATTY-ACIDS
• BACTERIAL TRANSLOCATION
• FUNCTIONAL RECOVERY
• ISCHEMIC BRAIN
• METABOLITES
• MICE
• SEX
• PERMEABILITY
• AMYLOIDOSIS

ObjectiveChronic systemic inflammation contributes to the pathogenesis of many age-related diseases. Although not well understood, alterations in the gut microbiota, or dysbiosis, may be responsible for age-related inflammation. @@@ MethodsUsing stroke as a disease model, we tested the hypothesis that a youthful microbiota, when established in aged mice, produces positive outcomes following ischemic stroke. Conversely, an aged microbiota, when established in young mice, produces negative outcomes after stroke. Young and aged male mice had either a young or an aged microbiota established by fecal transplant gavage (FTG). Mice were subjected to ischemic stroke (middle cerebral artery occlusion; MCAO) or sham surgery. During the subsequent weeks, mice underwent behavioral testing and fecal samples were collected for 16S ribosomal RNA analysis of bacterial content. @@@ ResultsWe found that the microbiota is altered after experimental stroke in young mice and resembles the biome of uninjured aged mice. In aged mice, the ratio of Firmicutes to Bacteroidetes (F:B), two main bacterial phyla in gut microbiota, increased approximate to 9-fold (p<0.001) compared to young. This increased F:B ratio in aged mice is indicative of dysbiosis. Altering the microbiota in young by fecal gavage to resemble that of aged mice (approximate to 6-fold increase in F:B ratio, p<0.001) increased mortality following MCAO, decreased performance in behavioral testing, and increased cytokine levels. Conversely, altering the microbiota in aged to resemble that of young (approximate to 9-fold decrease in F:B ratio, p<0.001) increased survival and improved recovery following MCAO. @@@ InterpretationAged biome increased the levels of systemic proinflammatory cytokines. We conclude that the gut microbiota can be modified to positively impact outcomes from age-related diseases. Ann Neurol 2018;83:23-36

• 出版日期2018-7