An ultrasensitive rapid immunocytotoxicity assay for detecting Clostridium difficile toxins

作者：He, Xianyun; Wang, Jufang; Steele, Jennifer; Sun, Xingmin; Nie, Weijia; Tzipori, Saul; Feng, Hanping^*

来源：Journal of Microbiological Methods, 2009, 78(1): 97-100.

DOI：10.1016/j.mimet.2009.04.007

• Clostridium difficile
• Toxins
• Diagnosis
• Cytotoxicity

We describe a novel ultrasensitive cell-based immunocytotoxicity assay for detecting Clostridium difficile toxin A and B. The assay is simple to perform with a turnaround time of approximately 3 h. It is particularly sensitive in detecting TcdA at a level less then 1 pg/ml. Using this assay, we were able to detect the presence of C, difficile toxins in the fecal and serum specimens of experimentally infected piglets.

• 出版日期2009-7
• 单位华南理工大学