Are skeletally mature female rats a suitable model to study osteoporosis?

作者：Netto Claudia Cardoso^*; Correia Vieira Vivian Cristine; Figueiredo Marinheiro Lizanka Paola; Agellon Sherry; Weiler Hope; Marostica Mario Roberto Jr

来源：Arquivos Brasileiros de Endocrinologia e Metabologia, 2012, 56(4): 259-264.

• Aging
• bone metabolism
• osteoporosis
• menopause

Objective: To analyze if female Wistar rats at 56 weeks of age are a suitable model to study osteoporosis. Materials and methods: Female rats with 6 and 36 weeks of age (n = 8 per group) were kept over a 20-week period and fed a diet for mature rodents complete in terms of Ca, phosphorous, and vitamin D. Excised femurs were measured for bone mass using dual-energy x-ray absorptiometry, morphometry, and biomechanical properties. The following serum markers of bone metabolism were analyzed: parathyroid hormone (PTH), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor kappa appa B ligand (RANKL), C-terminal peptides of type I collagen (CTX-I), total calcium, and alkaline phosphatase (ALP) activity. Results: Rats at 56 weeks of age showed important bone metabolism differences when compared with the younger group, such as, highest diaphysis energy to failure, lowest levels of OC, CTX-I, and ALP, and elevated PTH, even with adequate dietary Ca. Conclusion: Rats at 26-week-old rats may be too young to study age-related bone loss, whereas the 56-week-old rats may be good models to represent the early stages of age-related changes in bone metabolism. Arq Bras Endocrinol Metab. 2012;56(4):259-64

• 出版日期2012-6