Mutants of phospholipase A (pPLA-I) have a red light and auxin phenotype

作者：Effendi Yunus; Radatz Katrin; Labusch Corinna; Rietz Steffen; Wimalasekera Rinukshi; Helizon Hanna; Zeidler Mathias; Scherer Guenther F E^*

来源：Plant, Cell and Environment, 2014, 37(7): 1626-1640.

DOI：10.1111/pce.12278

• Arabidopsis
• gravitropism
• patatin-related phospholipase A
• phototropism
• phytochrome
• root coiling
• shade avoidance

pPLA-I is the evolutionarily oldest patatin-related phospholipase A (pPLA) in plants, which have previously been implicated to function in auxin and defence signalling. Molecular and physiological analysis of two allelic null mutants for pPLA-I [ppla-I-1 in Wassilewskija (Ws) and ppla-I-3 in Columbia (Col)] revealed pPLA-I functions in auxin and light signalling. The enzyme is localized in the cytosol and to membranes. After auxin application expression of early auxin-induced genes is significantly slower compared with wild type and both alleles show a slower gravitropic response of hypocotyls, indicating compromised auxin signalling. Additionally, phytochrome-modulated responses like abrogation of gravitropism, enhancement of phototropism and growth in far red-enriched light are decreased in both alleles. While early flowering, root coils and delayed phototropism are only observed in the Ws mutant devoid of phyD, the light-related phenotypes observed in both alleles point to an involvement of pPLA-I in phytochrome signalling.

• 出版日期2014-7