Ameloblastoma RNA profiling uncovers a distinct non-coding RNA signature

作者：Davanian Haleh; Balasiddaiah Anangi; Heymann Robert; Sundstrom Magnus; Redenstrom Poppy; Silfverberg Mikael; Brodin David; Sallberg Matti; Lindskog Sven; Weiner Carina Kruger; Chen Margaret^*

来源：Oncotarget, 2017, 8(3): 4530-4542.

DOI：10.18632/oncotarget.13891

• ameloblastoma
• biomarkers
• transcriptome
• ncRNA
• gene expression analysis

Ameloblastoma of the jaws remains the top difficult to treat odontogenic tumour and has a high recurrence rate. New evidence suggests that non-coding RNAs (ncRNAs) play a critical role in tumourgenesis and prognosis of cancer. However, ameloblastoma ncRNA expression data is lacking. Here we present the first report of ameloblastoma ncRNA signatures. A total of 95 ameloblastoma cases and a global array transcriptome technology covering > 285.000 full-length transcripts were used in this two-step analysis. The analysis first identified in a test cohort 31 upregulated ameloblastoma-associated ncRNAs accompanied by signalling pathways of cancer, spliceosome, mRNA surveillance and Wnt. Further validation in an independent cohort points out the long non-coding (lncRNAs) and small nucleolar RNA (snoRNAs): LINC340, SNORD116-25, SNORA11, SNORA21, SNORA47 and SNORA65 as a distinct ncRNA signature of ameloblastoma. Importantly, the presence of these ncRNAs was independent of BRAFV600E and SMO-L412F mutations, histology type or tumour location, but was positively correlated with the tumour size. Taken together, this study shows a systematic investigation of ncRNA expression of ameloblastoma, and illuminates new diagnostic and therapeutic targets for this invasive odontogenic tumour.

• 出版日期2017-1-17