科研之友
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摘要
Fluorescein has been coupled to the amino groups of the common nucleosides via a carbamoyl spacer to form a new type of conjugates. The corresponding phosphoramidites have been prepared with Npe and Npeoc protecting groups for application in oligonucleotide synthesis. Hybridizations have been studied in dependence of the fluorescing label as well as fluorescence quantum yields and fluorescence anisotropy effects.
- 出版日期1997
