科研之友
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摘要
Purpose: To investigate the antioxidant and antihaemolytic properties of the leaves of Laser trilubum grown in Gaduk, Iran.
Methods: The antioxidant and antihaemolytic activities of the hydroalcohol extract of L. trilobum L. leaf were investigated by haemoglobin-induced linoleic acid peroxidation, scavenging of 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, nitric oxide and hydrogen peroxide, as well as by assessment of reducing power and Fe(2+) chelating activity, using standard methods. Phenol and flavonoid contents were determined as gallic acid and quercetin equivalents, respectively.
Results: The extract showed antioxidant activity in some models, with 50 % inhibitory concentration (IC(50)) for DPPH radical-scavenging activity being 147.1 +/- 7.5 mu g ml(-1). The extract showed good nitric oxide-scavenging activity of between 0.1 and 1.6 mg ml(-1) (IC(50) = 517.7 +/- 23.1 vs. 20 +/- 0.01 mu g ml(-1) for quercetin), weak Fe(2+) chelating ability (IC(50), 906.9 +/- 37.8 mu g ml(-1)), and low antioxidant activity in haemoglobin-induced linoleic acid system. However, it was capable of scavenging hydrogen peroxide in a concentration-dependent manner while also exhibiting potent antihaemolytic activity against H(2)O(2) - induced haemolysis (IC(50), 169.6 +/- 6.9 mu g ml(-1)). Total phenolic content was 75 +/- 3 mg gallic acid equivalent/g and total flavonoid content 59.2 +/- 2.1 mg quercetin equivalent/g. The amounts of gallic acid, quercetin and rutin were 0.99 +/- 0.04, 0.63 +/- 0.01 and < 0.10 mu g/mg, respectively.
Conclusion: L. trilobum exhibited good but varying levels of antioxidant and antihaemolytic activities in nearly all the models studied, when compared with controls.
- 出版日期2010-10
