Multimodal imaging Gd-nanoparticles functionalized with Pittsburgh compound B or a nanobody for amyloid plaques targeting

作者：Pansieri Jonathan; Plissonneau Marie; Stransky Heilkron Nathalie; Dumoulin Mireille; Heinrich Balard Laurence; Rivory Pascaline; Morfin Jean Francois; Toth Eva; Saraiva Maria Joao; Allemann Eric; Tillement Olivier; Forge Vincent; Lux Francois; Marquette Christel^*

来源：Nanomedicine, 2017, 12(14): 1675-1687.

DOI：10.2217/nnm-2017-0079

• amyloid imaging
• amyloidoses
• gadolinium-based nanoparticles
• nanobody
• PIB

Aim: Gadolinium-based nanoparticles were functionalized with either the Pittsburgh compound B or a nanobody (B10AP) in order to create multimodal tools for an early diagnosis of amyloidoses. Materials & methods: The ability of the functionalized nanoparticles to target amyloid fibrils made of beta-amyloid peptide, amylin or Val30Met-mutated transthyretin formed in vitro or from pathological tissues was investigated by a range of spectroscopic and biophysics techniques including fluorescence microscopy. Results: Nanoparticles functionalized by both probes efficiently interacted with the three types of amyloid fibrils, with K-D values in 10 micromolar and 10 nanomolar range for, respectively, Pittsburgh compound B and B10AP nanoparticles. Moreover, they allowed the detection of amyloid deposits on pathological tissues. Conclusion: Such functionalized nanoparticles could represent promising flexible and multimodal imaging tools for the early diagnostic of amyloid diseases, in other words, Alzheimer's disease, Type 2 diabetes mellitus and the familial amyloidotic polyneuropathy.

• 出版日期2017-7
• 单位中国地震局