A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality

作者：Daigle, Tanya L.; Madisen, Linda; Hage, Travis A.; Valley, Matthew T.; Knoblich, Ulf; Larsen, Rylan S.; Takeno, Marc M.; Huang, Lawrence; Gu, Hong; Larsen, Rachael; Mills, Maya; Bosma-Moody, Alice; Siverts, La' Akea; Walker, Miranda; Graybuck, Lucas T.; Yao, Zizhen; Fong, Olivia; Thuc Nghi Nguyen; Garren, Emma; Lenz, Garreck H.; Chavarha, Mariya; Pendergraft, Julie; Harrington, James; Hirokawa, Karla E.; Harris, Julie A.; Nicovich, Philip R.; McGraw, Medea J.; Ollerenshaw, Douglas R.

来源：Cell, 2018, 174(2): 465-+.

DOI：10.1016/j.cell.2018.06.035

• NEURAL CIRCUITS
• IN-VIVO
• ADULT
• MICE
• CRE
• ORGANIZATION
• ACTIVATION
• EXCITATION
• NEURONS
• VOLTAGE

Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Here, we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targetedinsertion transgenesis in a wide range of neuronal types, along with additional advantage of a simplified breeding strategy compared to our first-generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of high-precision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain.

• 出版日期2018-7-12