Molecular Mechanisms Linking Autonomic Dysfunction and Impaired Cardiac Contractility in Critical Illness

作者：Ackland Gareth L^*; Whittle John; Toner Andrew; Machhada Asif; Del Arroyo Ana Gutierrez; Sciuso Alberto; Jenkins Nicholas; Dyson Alex; Struthers Richard; Sneyd J Robert; Minto Gary; Singer Mervyn; Shah Ajay M; Gourine Alexander V

来源：Critical Care Medicine, 2016, 44(8): E614-E624.

DOI：10.1097/CCM.0000000000001606

• autonomic dysfunction
• cardiac contractility
• G-protein-coupled receptor
• G-protein-coupled receptor kinase
• multiple organ dysfunction syndrome
• sepsis

Objectives: Molecular mechanisms linking autonomic dysfunction with poorer clinical outcomes in critical illness remain unclear. We hypothesized that baroreflex dysfunction alone is sufficient to cause cardiac impairment through neurohormonal activation of (nicotinamide adenine dinucleotide phosphate oxidase dependent) oxidative stress resulting in increased expression of G-protein-coupled receptor kinase 2, a key negative regulator of cardiac function.
Design: Laboratory/clinical investigations.
Setting: University laboratory/medical centers.
Subjects: Adult rats; wild-type/nicotinamide adenine dinucleotide phosphate oxidase subunit-2-deficient mice; elective surgical patients.
Interventions: Cardiac performance was assessed by transthoracic echocardiography following experimental baroreflex dyssino-aortic denervation) in rats and mice. Immunoblots assessed G-protein-coupled receptor recycling proteins expression in rodent cardiomyocytes and patient mononuclear leukocytes. In surgical patients, heart rate recovery after cardiopulmonary exercise testing, time/frequency measures of parasympathetic variables were related to the presence/absence of baroreflex dysdefined by spontaneous baroreflex sensitivity of <6 ms mm Hg-1). The associations of baroreflex dysfunction with intraoperative cardiac function and outcomes were assessed.
Measurements and Main Results: Experimental baroreflex dysfunction in rats and mice resulted in impaired cardiac contractility and upregulation of G-protein-coupled receptor kinase 2 expression. In mice, genetic deficiency of gp91 nicotinamide adenine dinucleotide phosphate oxidase subunit-2 prevented upregulation of G-protein-coupled receptor kinase 2 expression in conditions of baroreflex dysfunction and preserved cardiac function. Baroreflex dysfunction was present in 81 of 249 patients (32.5%) and was characterized by lower parasympathetic tone and increased G-protein-coupled receptor kinase 2 expression in mononuclear leukocytes. Baroreflex dysfunction in patients was also associated with impaired intraoperative cardiac contractility. Critical illness and mortality were more frequent in surgical patients with baroreflex dysrelative risk, 1.66 [95% CI, 1.16-2.39]; p = 0.006).
Conclusions: Reduced baroreflex sensitivity is associated with nicotinamide adenine dinucleotide phosphate oxidase subunit-2-mediated upregulation of G-protein-coupled receptor kinase 2 expression in cardiomyocytes and impaired cardiac contractility. Autonomic dysfunction predisposes patients to the development of critical illness and increases mortality.

• 出版日期2016-8