Design and validation of a bifunctional ligand display system for receptor targeting

作者：Chen LM; Zurita AJ; Ardelt PU; Giordano RJ; Arap W; Pasqualini R^*

来源：Chemistry & Biology, 2004, 11(8): 1081-1091.

DOI：10.1016/j.chembiol.2004.05.019

Here we developed a bacteriophage display particle designed to serve as a bifunctional entity that can target tumors while delivering an agent. We engineered a chimera phage vector containing a pIII-displayed alpha(v) integrins-targeting moiety and a pVIII-displayed streptavidin binding adaptor moiety. By using the chimeric phage particle, targeting of alpha(v) integrins on cells in culture and tumor-related blood vessels was shown through different applications, including luminescent quantum dots localization, surface plasmon resonance-based binding detection, and an in vivo tumor model. The strategy validated here will accelerate the discovery and characterization of receptor-ligand binding events in high throughput, and cell-specific delivery of diagnostics or therapeutics to organs of choice without the need for chemical conjugation.

• 出版日期2004-8