Anti-tumor and immunomodulatory activity of selenium (Se)-polysaccharide from Se-enriched Grifola frondosa

作者：Mao, Guang-Hua; Ren, Yi; Li, Qian; Wu, Hui-Yu; Jin, Dun; Zhao, Ting; Xu, Cai-Quan; Zhang, Deng-Hong; Jia, Qing-Dong; Bai, Yan-Peng; Yang, Liu-Qing^*; Wu, Xiang-Yang

来源：International Journal of Biological Macromolecules, 2016, 82: 607-613.

DOI：10.1016/j.ijbiomac.2015.10.083

• Se-enriched Grifola frondosa
• Polysaccharide
• Characterization
• Anti-tumor
• Immunomodulatory

A polysaccharide termed Se-GP11 was extracted and purified from Se-enriched Grifola frondosa in our previous study. This study investigated the characterization, anti-tumor and immunomodulatory activity of Se-GP11. The results showed that Se-GP11 was composed of mannose, glucose and galactose with a molar ratio of 1:4.91:2.41. The weight-average molecular weight (M-w) and weight-average mean square radius (R-w) of Se-GP11 in 0.1 M sodium chloride solution were 3.3 x 10(4) Da and 32.8 nm. Se-GP11 existed as a globular conformation with random coil structure. Se-GP11 had no anti-tumor activity against HepG-2 cells in vitro, and it significantly inhibited the growth of Heps tumor in vivo. Se-GP11 increased the relatively thymus and spleen weights as well as serum necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2) levels. In addition, Se-GP11 promoted the phagocytosis and NO production of RAW264.7 as compared with that of the normal control group. The results revealed that the Se-GP11 may exhibit the anti-tumor through improving immunologic function of the tumor bearing mice.

• 出版日期2016-1
• 单位江苏大学