Nardosinone Protects H9c2 Cardiac Cells from Angiotensin Ⅱ-induced Hypertrophy

作者:杜萌; 黄坤; 高路; 杨柳; 王文硕; 王博; 黄恺; 黄丹
来源:Journal of Huazhong University of Science and Technology-Medical Sciences, 2013, 33(06): 822-826.

摘要

Pathological cardiac hypertrophy induced by angiotensinⅡ(AngⅡ) can subsequently give rise to heart failure,a leading cause of mortality.Nardosinone is a pharmacologically active compound extracted from the roots of Nardostachys chinensis,a well-known traditional Chinese medicine.In order to investigate the effects of nardosinone on AngⅡ-induced cardiac cell hypertrophy and the related mechanisms,the myoblast cell line H9c2,derived from embryonic rat heart,was treated with nardosinone(25,50,100,and 200μmol/L) or AngⅡ(1μmol/L).Then cell surface area and mRNA expression of classical markers of hypertrophy were detected.The related protein levels in PI3K/Akt/mTOR and MEK/ERK signaling pathways were examined by Western blotting.It was found that pretreatment with nardosinone could significantly inhibit the enlargement of cell surface area induced by AngⅡ.The mRNA expression of ANP,BNP andβ-MHC was obviously elevated in AngⅡ-treated H9c2 cells,which could be effectively blocked by nardosinone at the concentration of 100μmol/L.Further study revealed that the protective effects of nardosinone might be mediated by repressing the phosphorylation of related proteins in PI3K/Akt and MEK/ERK signaling pathways.It was suggested that the inhibitory effect of nardosinone on AngⅡ-induced hypertrophy in H9c2 cells might be mediated by targeting PI3K/Akt and MEK/ERK signaling pathways.