A novel fluorescent adhesive-assisted biomimetic mineralization

作者：Wang, Zhe; Ouyang, Yan; Wu, Zhifang; Zhang, Leiqing; Shao, Changyu; Fan, Jiayan; Zhang, Ling; Shi, Ying; Zhou, Zihuai; Pan, Haihua; Tang, Ruikang; Fu, Baiping^*

来源：Nanoscale, 2018, 10(40): 18980-18987.

DOI：10.1039/c8nr02078g

• AMORPHOUS CALCIUM-PHOSPHATE
• PRECURSOR PILP PROCESS
• INTRAFIBRILLAR MINERALIZATION
• COLLAGEN
• REMINERALIZATION
• ENAMEL
• NUCLEATION
• BONE
• HYDROXYAPATITE
• BIOMINERALIZATION

We propose a novel fluorescent adhesive-assisted biomimetic mineralization strategy, based on which 1 wt% of sodium fluorescein and 25 wt% of polyacrylic acid stabilized amorphous calcium phosphate (PAA-ACP) nanoparticles were incorporated into a mild self-etch adhesive (Clearfil S3 Bond) as a fluorescent mineralizing adhesive. The characterization of the PAA-ACP nanoparticles indicates that they were spherical particles clustered together, each particle with a diameter of approximately 20-50 nm, in a metastable phase with two characteristic absorption peaks (1050 cm(-1) and 580 cm(-1)). Our results suggest that the fluorescent mineralizing adhesive was non-cytotoxic with minimal esthetic interference and its fluorescence intensity did not significantly decrease within 6 months. Our data reveal that the fluorescent mineralizing adhesive could induce the extra- and intra-fibrillar remineralization of the reconstituted type I collagen, the demineralized enamel and dentin substrate. Our data demonstrate that a novel fluorescent adhesive-assisted biomimetic mineralization strategy will pave the way to design and produce anti-carious materials for the prevention of dental caries.

• 出版日期2018-10-28
• 单位浙江大学; 南昌大学附属口腔医院