Long-term protection from SARS coronavirus infection conferred by a single immunization with an attenuated VSV-based vaccine

作者：Kapadia SU; Rose JK^*; Lamirande E; Vogel L; Subbarao K; Roberts A

来源：Virology, 2005, 340(2): 174-182.

DOI：10.1016/j.virol.2005.06.016

• intranasal
• neutralizing antibodies
• protective immunity
• spike

Although the recent SARS coronavirus (SARS-CoV) that appeared in 2002 has now been contained, the possibility of re-emergence of SARS-CoV remains. Due to the threat of re-emergence, the overall fatality rate of similar to 10%, and the rapid dispersion of the virus via international travel, viable vaccine candidates providing protection from SARS are clearly needed. We developed an attenuated VSV recombinant (VSV-S) expressing the SARS coronavirus (SARS-CoV) spike (S) protein. In cells infected with this recombinant, S protein was synthesized, glycosylated at approximately 17 Asn residues, and transported via the Golgi to the cell surface. Mice vaccinated with VSV-S developed SARS-neutralizing antibody and were able to control a challenge with SARS-CoV performed at I month or 4 months after a single vaccination. We also demonstrated, by passive antibody transfer, that the antibody response induced by the vaccine was sufficient for controlling SARS-CoV infection. A VSV-vectored SARS vaccine could have significant advantages over other SARS vaccine candidates described to date.

• 出版日期2005-9-30