Assessment of free-radical scavenging activity of Gypsophila pilulifera: assay-guided isolation of verbascoside as the main active component

作者：Chima Navdeep K; Nahar Lutfun; Majinda Runner R T; Celik Sezin; Sarker Satyajit D^*

来源：Brazilian Journal of Pharmacognosy, 2014, 24(1): 38-43.

DOI：10.1590/0102-695X20142413391

• Gypsophila pilulifera
• Free-radical scavenger
• Solid-phase extraction
• High-performance liquid chromatography (HPLC)
• Phenylethanoid
• Verbascoside

Gypsophila pilulifera, Boiss %26 Heldr, Caryophyllaceae, is a perennial medicinal herb that grows in the-southwestern region of Turkey. Except for only one report on the isolation-of cytotoxic. saponins from the underground parts of G. pilulifera, there are no published thorough phytochemical or bioactivity studies on this specie. In the present study; the free-radical Scavenging activity of extracts and fractions of the-stems of G. pilulifera was evaluated, using a. slightly modified and more-precise-version of the 2,2-diphenyl-1-picrylhydrazyl (DRPH) assay, :reported here for the first time. The DPPH assay-guided HPLC-PDA-purification of the active solid-phase extraction fraction (50% methanol in water) of the methanolic-extract exhibited verbascoside as the main free-radical Scavenger present in this species. The structure of this active compound was resolved by spectroscopy, and the free-radical scavenging potential of verbascoside was determined.

• 出版日期2014-2