Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 Cells In Vitro and In Vivo

作者：Bingula Rea; Dupuis Carmen; Pichon Chantal; Berthon Jean Yves; Filaire Marc; Pigeon Lucie; Filaire Edith

来源：Journal of Oncology, 2016, 2016: 8162952.

DOI：10.1155/2016/8162952

<jats:p>We investigated the effects of betaine, C-phycocyanin (C-PC), and their combined use on the growth of A549 lung cancer both<jats:italic>in vitro</jats:italic>and<jats:italic>in vivo.</jats:italic>When cells were coincubated with betaine and C-PC, an up to 60% decrease in viability was observed which is significant compared to betaine (50%) or C-PC treatment alone (no decrease). Combined treatment reduced the stimulation of NF-<jats:italic>κ</jats:italic>B expression by TNF-<jats:italic>α</jats:italic>and increased the amount of the proapoptotic p38 MAPK. Interestingly, combined treatment induced a cell cycle arrest in G<jats:sub>2</jats:sub>/M phase for ~60% of cells.<jats:italic>In vivo</jats:italic>studies were performed in pathogen-free male nude rats injected with A549 cells in their right flank. Their daily food was supplemented with either betaine, C-PC, both, or neither. Compared to the control group, tumour weights and volumes were significantly reduced in either betaine- or C-PC-treated groups and no additional decrease was obtained with the combined treatment. This data indicates that C-PC and betaine alone may efficiently inhibit tumour growth in rats. The synergistic activity of betaine and C-PC on A549 cells growth observed<jats:italic>in vitro</jats:italic>remains to be further confirmed<jats:italic>in vivo.</jats:italic>The reason behind the nature of their interaction is yet to be sought.</jats:p>

• 出版日期2016