科研之友
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摘要
We report a modified method of screening for point mutations using a PCR approach based upon the sensitivity of PCR to the 3' terminus of the primer. This method provides a sensitive screen when using either plasmid DNA or bacterial cell lysates. We have optimized the technique for general use to allow rapid screening of mutants with good discrimination. Unlike previous similar methods, this technique has no inherent limitation in primer design on the 3'-terminal base chosen.
- 出版日期1992-1
