Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema lectin and chemical modification properties on its mannose-binding sites

作者：Liu Bo; Xu Xiao chao; Cheng Yan; Huang Jian; Liu Yan hong; Liu Zhen; Min Ming wei; Bian He jiao; Chen Jing; Bao Jin ku^*

来源：BMB Reports, 2008, 41(5): 369-375.

• apoptosis
• chemical modification
• mannose-binding sites
• Polygonatum cyrtonema lectin (PCL)

Polygonatum cyrtonema Lectin (PCL), which is classified as a monocot mannose-binding lectin, has received great regards for its uniquely biological activities and potentially medical applications in cancer cells. This paper was initially aimed to study apoptosis of PCL on Hela cells. Thus, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MM method was carried out Through observation of cell morphologic changes and Lactate dehydrogenase (LDH) activity-based cytotoxicity assays, PCL induced HeLa cell apoptosis in a close-dependent manner. To further gain structural basis, multiple alignments, homology modeling and docking experiments were performed to analyze the correlation between its biological activities and mannose-binding sites. Eventually, considering docking data, chemical modification properties on the three mannose-binding sites were analyzed by a series of biological experiments (e.g., hemagglutinating and mitogenic activity assays, fluorescence and Circular Dichrosim (CD) spectroscopy) to profoundly identify the role of some key amino acids in the structure-function relationship of PCL.

• 出版日期2008-5-31
• 单位河北医科大学; 沈阳药科大学; 四川大学