EXPRESSION OF MESSENGER-RNA OF MURINE BONE-RELATED PROTEINS IN ECTOPIC BONE INDUCED BY MURINE BONE MORPHOGENETIC PROTEIN-4

作者：HIROTA S; TAKAOKA K; HASHIMOTO J; NAKASE T; TAKEMURA T; MORII E; FUKUYAMA A; MORIHANA K; KITAMURA Y; NOMURA S

来源：Cell and Tissue Research, 1994, 277(1): 27-32.

DOI：10.1007/BF00303077

• OSTEONECTIN
• OSTEOPONTIN
• OSTEOCALCIN CHONDROGENESIS
• OSTEOGENESIS
• BONE MORPHOGENETIC PROTEIN-4
• MOUSE

To determine whether a system of ectopic bone formation induced by osteosarcoma-derived bone-inducing substance (bone morphogenetic protein-4) can be used as a model of developing bone at the molecular level, we studied the expression of bone-related protein mRNAs in the process of ectopic bone formation using non-radioisotopic in situ hybridization. Osteonectin mRNA was detected in fibroblast-like cells, which are similar to periosteal cells from the early to middle stages of bone development. The proportion of osteonectin mRNA-expressing cells was greater than that of osteopontin mRNA-expressing cells in hypertrophic chondrocytes and osteoblast-like cells. In contrast, osteopontin mRNA was localized in a limited population of hypertrophic chondrocytes, a single layer of osteoblast-like cells adjacent to the bone trabeculae in the middle stage of bone formation, and in a limited subset of osteocytes in the late stage. A strong osteocalcin mRNA signal was detected in osteoblast-like cells from the middle to late stages and in a limited subset of osteocytes in the late stage of bone development. Since the sequential gene expression pattern of bone-related proteins in the present system is comparable to that in embryonic osteogenesis, this system may be useful as a model for studying gene expression in osteogenesis.

• 出版日期1994-7