A sensitive and efficient method for trace analysis of some phenolic compounds using simultaneous derivatization and air-assisted liquid-liquid microextraction from human urine and plasma samples followed by gas chromatography-nitrogen phosphorous detection

作者：Farajzadeh Mir Ali^*; Mogaddam Mohammad Reza Afshar; Nabil Ali Akbar Alizadeh

来源：Biomedical Chromatography, 2015, 29(12): 1921-1931.

DOI：10.1002/bmc.3517

• phenolic compounds
• air-assisted liquid-liquid microextraction
• 1-flouro-2
• 4-dinitrobenzene
• gas chromatography
• nitrogen-phosphorous detector

In present study, a simultaneous derivatization and air-assisted liquid-liquid microextraction method combined with gas chromatography-nitrogen phosphorous detection has been developed for the determination of some phenolic compounds in biological samples. The analytes are derivatized and extracted simultaneously by a fast reaction with 1-flouro-2,4-dinitrobenzene under mild conditions. Under optimal conditions low limits of detection in the range of 0.05-0.34 ng mL(-1) are achievable. The obtained extraction recoveries are between 84 and 97% and the relative standard deviations are less than 7.2% for intraday (n = 6) and interday (n = 4) precisions. The proposed method was demonstrated to be a simple and efficient method for the analysis of phenols in biological samples.

• 出版日期2015-12