Wild Birds, Frequent Carriers of Extended-Spectrum beta-Lactamase (ESBL) Producing Escherichia coli of CTX-M and SHV-12 Types

作者：Alcala Leticia; Andrea Alonso Carla; Simon Carmen; Gonzalez Esteban Chabier; Oros Jesus; Rezusta Antonio; Ortega Carmelo; Torres Carmen^*

来源：Microbial Ecology, 2016, 72(4): 861-869.

DOI：10.1007/s00248-015-0718-0

• ESBL
• CMY-2
• PMQR
• Escherichia coli
• Wild birds
• Spain

To get a better insight into the role of birds as reservoirs of extended-spectrum beta-lactamase (ESBL) and plasmidic AmpC beta-lactamase (pAmpC) Escherichia coli producers, 100 fecal samples belonging to 15 different wild avian species from Northern Spain were analyzed. Cefotaxime-resistant (CTXR) E. coli isolates were identified in 16 of the 100 tested birds, which corresponded to 9 animal species (Gyps fulvus-griffon vulture, Larus michahellis-yellow-legged gull, Milvus migrans-black kite, Milvus milvus-red kite, Ciconia ciconia-white stork, Sturnus unicolor-spotless starling, Aquila chrysaetos-golden eagle, Cuculus canorus-common cuckoo, Tyto alba-barn owl). Fifteen isolates harbored ESBL or pAmpC-encoding genes (number of isolates): bla(SHV-12) (9), bla(CTX-M-1) (3), bla(CTX-M-14) (2), and bla(CMY-2) (1). The last CTXR isolate presented a -42-point-mutation in the chromosomal ampC promoter. Eleven out of 15 ESBL/pAmpC E. coli isolates were multiresistant (most common resistance phenotype: beta-lactams-quinolones-tetracycline-sulfamethoxazole/trimethoprim). A plasmid-mediated quinolone resistance determinant (qnrS1) was identified in one E. coli from a barn owl. High genetic diversity was observed among ESBL/pAmpC E. coli isolates, with 12 different sequence types (STs), including several strains of STs frequently detected among human clinical isolates (ST38/D, ST131/B2, ST155/B1, ST10/A). The ST131 isolate belonged to the emergent ciprofloxacin-resistant H30R subclone. This study reveals a high percentage of bird as carriers of ESBL/pAmpC E. coli isolates in Spain, highlighting the elevated rate among storks, kites, and vultures. Wild birds can contribute to the global spread of ESBL/pAmpC-producing E. coli in natural ecosystems.

• 出版日期2016-11