Avoiding the oligomeric state: alpha B-crystallin inhibits fragmentation and induces dissociation of apolipoprotein C-II amyloid fibrils

作者：Binger Katrina J; Ecroyd Heath; Yang Shuo; Carver John A; Howlett Geoffrey J; Griffin Michael D W^*

来源：The FASEB Journal, 2013, 27(3): 1214-1222.

DOI：10.1096/fj.12-220657

• protein misfolding
• aggregation
• molecular chaperone
• heat-shock protein

The in vivo aggregation of proteins into amyloid fibrils suggests that cellular mechanisms that normally prevent or reverse this aggregation have failed. The small heat-shock molecular chaperone protein alpha B-crystallin (alpha B-c) inhibits amyloid formation and colocalizes with amyloid plaques; however, the physiological reason for this localization remains unexplored. Here, using apolipoprotein C-II (apoC-II) as a model fibril-forming system, we show that alpha B-c binds directly to mature amyloid fibrils (K-d 5.4 +/- 0.5 mu M). In doing so, alpha B-c stabilized the fibrils from dilution-induced fragmentation, halted elongation of partially formed fibrils, and promoted the dissociation of mature fibrils into soluble monomers. Moreover, in the absence of dilution, the association of alpha B-c with apoC-II fibrils induced a 14-fold increase in average aggregate size, resulting in large fibrillar tangles reminiscent of protein inclusions. We propose that the binding of alpha B-c to fibrils prevents fragmentation and mediates the lateral association of fibrils into large inclusions. We further postulate that transient interactions of apoC-II with alpha B-c induce a fibril-incompetent monomeric apoC-II form, preventing oligomerization and promoting fibril dissociation. This work reveals previously unrecognized mechanisms of alpha B-c chaperone action in amyloid assembly and fibril dynamics, and provides a rationale for the in vivo colocalization of small heat-shock proteins with amyloid deposits.-Binger, K. J., Ecroyd, H., Yang, S., Carver, J. A., Howlett, G. J., Griffin, M. D. W. Avoiding the oligomeric state: alpha B-crystallin inhibits fragmentation and induces dis-sociation of apolipoprotein C-II amyloid fibrils. FASEB J. 27, 1214-1222 (2013). www.fasebj.org

• 出版日期2013-3