A Three-Locus, PCR-based Method for Forensic Identification of Plant Material

作者：Srivastava Tushar; Wu Michael; Kakhnovich Julia; Waithaka Bridgit; Lents Nathan H.^*

来源：Journal of Forensic Sciences, 2018, 63(4): 1252-1260.

DOI：10.1111/1556-4029.13715

• forensic science
• botany
• forensic botany
• forensic biology
• DNA barcodes
• DNA barcoding
• pollen
• plants
• polymerase chain reaction

Plant residue is currently an underutilized resource in forensic investigations despite the fact that many crime scenes, as well as suspects and victims, harbor plant-derived residue that could be recovered and analyzed. Notwithstanding the considerable skill of forensic botanists, current methods of species determination could benefit from tools for DNA-based species identification. However, DNA barcoding in plants has been hampered by sequence complications in the plant genome. Following a database search for usable barcodes, broad-spectrum primers were designed and utilized to amplify and sequence the rbcL, trnL-F, and rrn18 genetic loci from a variety of household plants. Once obtained, these DNA sequences were used to design species-targeted primers that could successfully discriminate the source of plant residue from among the 21 species tested.

• 出版日期2018-7