Analysis of Gene Expression in a Human-derived Glial Cell Line Exposed to 2.45 GHz Continuous Radiofrequency Electromagnetic Fields

作者：Sakurai Tomonori; Kiyokawa Tomoko; Narita Eijiro; Suzuki Yukihisa; Taki Masao; Miyakoshi Junji^*

来源：Journal of Radiation Research, 2011, 52(2): 185-192.

DOI：10.1269/jrr.10116

Radiofrequency electromagnetic fields/Human-derived cells/DNA microarray/Polymerase chain reaction.
The increasing use of mobile phones has aroused public concern regarding the potential health risks of radiofrequency (RF) fields. We investigated the effects of exposure to RF fields (2.45 GHz, continuous wave) at specific absorption rate (SAR) of 1, 5, and 10 W/kg for 1, 4, and 24 h on gene expression in a normal human glial cell line, SVGp12, using DNA microarray. Microarray analysis revealed 23 assigned gene spots and 5 non-assigned gene spots as prospective altered gene spots. Twenty-two genes out of the 23 assigned gene spots were further analyzed by reverse transcription-polymerase chain reaction to validate the results of microarray, and no significant alterations in gene expression were observed. Under the experimental conditions used in this study, we found no evidence that exposure to RF fields affected gene expression in SVGp12 cells.

• 出版日期2011-3