Gene expression and phenotypic analyses of transgenic Chinese cabbage over-expressing the cold tolerance gene, BrCSR

作者：Yu Jae Gyeong; Lee Gi Ho; Lee Sang Choon; Park Young Doo^*

来源：Horticulture Environment and Biotechnology, 2014, 55(5): 415-422.

DOI：10.1007/s13580-014-0054-1

• gene co-expression network
• gene ontology
• quantitative real-time RT-PCR

The goal of this study is to characterize the BrCSR (B. rapa Cold Stress Resistance) gene and to analyze the expression pattern of genes related to cold response under low temperatures in Chinese cabbage. A transgenic Chinese cabbage line was generated using expression vector pSL101 containing the BrCSR full length CDS. Five transgenic plants of T-1-progeny that were selected by PCR and southern hybridization showed approximately 2-fold higher expression than the wild-type control under cold stress conditions. These transgenic plants showed less susceptibility to chilling injury compared to the control. To evaluate genes that are functionally related to BrCSR and cold-responsive genes, a gene co-expression network had been preferentially constructed, and then B. rapa 135K cDNA microarray was subsequently analyzed. BrCSR was strongly associated with PDP1, NYC1, and CYP72A11, which are involved in the cold stress tolerance response. Expression levels of genes related to the biosynthesis of succinate and thiamine, which have reported to be associated with cold tolerance, significantly changed in the gene co-expression network. Taken together, these results indicated that BrCSR plays a significant role in the adaptation of plants to low temperature conditions.

• 出版日期2014-10