Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing

作者：Loh Evelyn Yun Xi; Mohamad Najwa; Fauzi Mh Busra; Min Hwei Ng; Shiow Fern Ng; Amin Mohd Cairul Iqbal Mohd

来源：Scientific Reports, 2018, 8(1): 2875.

DOI：10.1038/s41598-018-21174-7

<jats:title>Abstract</jats:title><jats:p>Bacterial cellulose (BC)/acrylic acid (AA) hydrogel has successfully been investigated as a wound dressing for partial-thickness burn wound. It is also a promising biomaterial cell carrier because it bears some resemblance to the natural soft tissue. This study assessed its ability to deliver human epidermal keratinocytes (EK) and dermal fibroblasts (DF) for the treatment of full-thickness skin lesions. <jats:italic>In vitro</jats:italic> studies demonstrated that BC/AA hydrogel had excellent cell attachment, maintained cell viability with limited migration, and allowed cell transfer. <jats:italic>In vivo</jats:italic> wound closure, histological, immunohistochemistry, and transmission electron microscopy evaluation revealed that hydrogel alone (HA) and hydrogel with cells (HC) accelerated wound healing compared to the untreated controls. Gross appearance and Masson’s trichrome staining indicated that HC was better than HA. This study suggests the potential application of BC/AA hydrogel with dual functions, as a cell carrier and wound dressing, to promote full-thickness wound healing.</jats:p>

• 出版日期2018-2-13