Access to Follicular Dendritic Cells Is a Pivotal Step in Murine Chronic Lymphocytic Leukemia B-cell Activation and Proliferation

作者：Heinig Kristina; Gaetjen Marcel; Grau Michael; Stache Vanessa; Anagnostopoulos Ioannis; Gerlach Kerstin; Niesner Raluca A; Cseresnyes Zoltan; Hauser Anja E; Lenz Peter; Hehlgans Thomas; Brink Robert; Westermann Joerg; Doerken Bernd; Lipp Martin; Lenz Georg; Rehm Armin; Hoepken Uta E

来源：Cancer Discovery, 2014, 4(12): 1448-1465.

DOI：10.1158/2159-8290.CD-14-0096

In human chronic lymphocytic leukemia (CLL) pathogenesis, B-cell antigen receptor signaling seems important for leukemia B-cell ontogeny, whereas the microenvironment influences B-cell activation, tumor cell lodging, and provision of antigenic stimuli. Using the murine E mu-Tcl1 CLL model, we demonstrate that CXCR5-controlled access to follicular dendritic cells confers proliferative stimuli to leukemia B cells. Intravital imaging revealed a marginal zone B cell-like leukemia cell trafficking route. Murine and human CLL cells reciprocally stimulated resident mesenchymal stromal cells through lymphotoxin-beta-receptor activation, resulting in CXCL13 secretion and stromal compartment remodeling. Inhibition of lymphotoxin/lymphotoxin-beta-receptor signaling or of CXCR5 signaling retards leukemia progression. Thus, CXCR5 activity links tumor cell homing, shaping a survival niche, and access to localized proliferation stimuli. %26lt;br%26gt;SIGNIFICANCE: CLL and other indolent lymphoma are not curable and usually relapse after treatment, a process in which the tumor microenvironment plays a pivotal role. We dissect the consecutive steps of CXCR5-dependent tumor cell lodging and LT beta R-dependent stroma-leukemia cell interaction; moreover, we provide therapeutic solutions to interfere with this reciprocal tumor-stroma cross-talk.

• 出版日期2014-12
• 单位河北医科大学