Kinetic Studies of Dextransucrase Enzyme Reactions on a Substrate- or Enzyme-Immobilized 27 MHz Quartz Crystal Microbalance

作者：Nihira Takanori; Mori Toshiaki^*; Asakura Megumi; Okahata Yoshio

来源：Langmuir, 2011, 27(6): 2107-2111.

DOI：10.1021/la104550m

Catalytic elongation by dextransucrase (DSase) was monitored directly on a dextran-acceptor- or DSase-immobilized 27 MHz quartz crystal microbalance (QCM) Kinetic parameters for the binding of the enzyme to the dextran acceptor (k(on), k(off), and K(d)) and enzymatic elongation in the presence of a sucrose Monomer (K(m) for sucrose and k(cat)) were determined. The kinetic parameters obtained by both methods were consistent.

• 出版日期2011-3-15