Analysis of Dongbi Longan with Isoenzyme and RAPD Technologies
3rd International Symposium on Longan, Lychee and other Fruit Trees in Sapindaceae Family, 2008-8-25 ~ 2008-8-29, pp 103-109, 2010
Longan (Dimocarpus longan Lour.) is one of the most important subtropical fruit trees in Fujian province. Due to little genetic background, the development of longan resources study was restricted. Random amplified polymorphic DNA (RAPD) and POD isoenzyme techniques were used to study the genetic polymorphism and relationship among strains of Dongbi longan. Twenty two arbitrary 10mer primers were used in RAPD analysis of 13 Dongbi longan strains. A total of 124 bands were amplified from 13 samples, each primer pair produces 3 (S299) to 9 (S60) bands, and the average bands were 5.64. The RAPD results showed that the genetic comparability was significantly high and there was high genetic stability among 13 strains, but genes varied with the time and environment. Compared to RAPD results, there was no significant difference in POD isoenzyme among 13 samples from POD isoenzyme gel. According to the results, we concluded that RAPD technology is more suitable than POD isoenzyme technology for longan germplasm resources analysis.
POD isoenzyme; molecular markers; clustering analysis; genetic diversity