Development and evaluation of consolidated bioprocessing yeast for ethanol production from ionic liquid-pretreated bagasse

作者：Amoah Jerome; Ishizue Naoya; Ishizaki Miki; Yasuda Misa; Takahashi Kenji; Ninomiya Kazuaki; Yamada Ryosuke; Kondo Akihiko; Ogino Chiaki^*

来源：Bioresource Technology, 2017, 245: 1413-1420.

DOI：10.1016/j.biortech.2017.05.171

• Bioethanol
• Lignocellulose
• Cocktail delta-integration
• Cell surface display
• Enzyme activity

This work aimed to study the use of consolidated bioprocess (CBP) yeast expressing five cellulase genes (BGL, XYNII, EGII, CBHI and CBHII) for ethanol production from ionic liquid-pretreated bagasse and Laubholz unbleached Kraft pulp (LUKP). A proposed screening method shows that the optimal cellulase ratio varies for each biomass substrate, and thus it is essential to breed CBP yeast having optimal cellulase-displaying ratio for the target biomass. CBP yeast specialized towards bagasse produced 0.93 g/l ethanol whiles that for LUKP produced 0.71 g/l ethanol, which is approximately 4 and 2-fold, respectively, higher than that of the wild type. The cell-surface displayed enzymes synergistically contributed to the degradation of the biomass. The developed CBP yeast is a potential cheap source for consolidated bioprocessing of ethanol and the proposed screening method can be used for matching CBP yeast to a target biomass.

• 出版日期2017-12