N-acetyl-cysteine (NAC) attenuates LPS-induced maternal and amniotic fluid oxidative stress and inflammatory responses in the preterm gestation

作者：Awad Nibal; Khatib Nizar; Gin**erg Yuval; Weiner Zeev; Maravi Nir; Thaler Israel; Ross Michael G; Itsokovitz Eldor Joseph; Beloosesky Ron^*

来源：American Journal of Obstetrics and Gynecology, 2011, 204(5): 450.e15.

DOI：10.1016/j.ajog.2011.01.030

• amniotic fluid
• inflammation
• maternal infection
• N-acetyl-cysteine
• rat

OBJECTIVE: Maternal infection is associated with oxidative stress and inflammation. We sought to determine whether N-acetyl-cysteine can decrease maternal oxidative stress and the inflammatory response in preterm gestation.
STUDY DESIGN: Pregnant rats 16 days, were treated with (1) lipopolysaccharide, (2) N-acetyl-cysteine 120 minutes after lipopolysaccharide, or (3) saline solution (intraperitoneal). Six hours after lipopolysaccharide administration, serum lipid peroxide formation (LPO), tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta levels in maternal serum and amniotic fluid were determined.
RESULTS: Lipopolysaccharide significantly increased maternal serum lipid peroxide formation (24-118.5 nmol/mL; P < .05), and maternal serum and amniotic fluid tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta. N-acetyl-cysteine treatment after lipopolysaccharide significantly attenuated lipid peroxide formation (47.5 nmol/mL) and proinflammatory cytokines response in maternal serum and amniotic fluid.
CONCLUSION: Maternal and amniotic fluid oxidative stress and inflammatory stimulation are attenuated by N-acetyl-cysteine even when administered after lipopolysaccharide. These results suggest that N-acetyl-cysteine may protect the fetus from adverse sequelae associated with inflammatory stimulation.

• 出版日期2011-5