Mouse testis-brain RNA-binding protein (TB-RBP): expression, purification and crystal X-ray diffraction

作者：Pascal JM; Chennathukuzhi VM; Hecht NB; Robertus JD^*

来源：Acta Crystallographica Section D-Biological Crystallography, 2001, 57: 1692-1694.

DOI：10.1107/S0907444901014548

TB-RBP (testis-brain RNA-binding protein) is a mouse RNA-binding protein that controls the temporal and spatial expression of mRNA. Found most abundantly in brain and male germ cells in the testis, TB-RBP is known to suppress the translation of specific testicular and brain mRNAs as part of cell development. TB-RBP- mRNA complexes can bind microtubules and thereby facilitate RNA translocation. Translin is the human orthologue of TB-RBP which binds to single-stranded ends of DNA sequences in breakpoint regions of chromosomal translocations. TB-RBP/translin has been crystallized in space group P2(1)2(1)2. The expression, purification, and crystallization of TB-RBP are described as well as preliminary X-ray diffraction data. The multimeric state of TB-RBP is addressed using dynamic light-scattering results.

• 出版日期2001-11