A novel ncRNA gene from mouse chromosome 5 trans-splices with Dmrt1 on chromosome 19

作者：Zhang, Lei; Lu, Heng; Xin, Dazhuan; Cheng, Hanhua; Zhou, Rongjia^*

来源：Biochemical and Biophysical Research Communications, 2010, 400(4): 696-700.

DOI：10.1016/j.bbrc.2010.08.130

• Sex determination
• Trans-splicing
• Double-sex
• ncRNA
• Mouse

Dmrt1 (Dsx- and Mab3-related transcription factor-1), a conserved transcription factor in different phyla, is a key regulator in sex determination. Here, we report the novel ncRNA gene Dmr (Dmrt1-related gene), from mouse chromosome 5 that trans-splices with Dmrt1 from chromosome 19 to generate a Dmrt1 protein that lacks the C-terminus. Dmr is mouse and rat specific, and the surrounding genes are also conserved in both species. Dmr is alternatively spliced, and three isoforms, Dmr a, b and c, are detected in the testis. Further, Dmr serves mainly as a 3' UTR, promotes trans-splicing and down-regulates the Dmrt1 protein. These results suggest that Dmr might play a negative regulatory role for Dmrt1 in male sexual development.

• 出版日期2010-10-1
• 单位武汉大学