DETECTION OF MESSENGER-RNA ENCODING AN ANTIBACTERIAL-METALLOPROTEIN (MPII) BY IN-SITU HYBRIDIZATION WITH A CDNA PROBE GENERATED BY POLYMERASE CHAIN-REACTION IN THE WORM NEREIS-DIVERSICOLOR

作者：SALZETR**EILLON B^*; RENTIERDELRUE F; DHAINAUT A

来源：Cellular and Molecular Biology, 1993, 39(1): 105-114.

• PCR
• IN-SITU HYBRIDIZATION
• ANTIBACTERIAL-METALLOPROTEIN
• HEMERYTHRIN-MYOHEMERYTHRIN FAMILY

Based on partial amino acid sequence of an antibacterial-metalloprotein (MPII) consisting of a N-terminal fragment(1-33 th) and an 43 amino acids long internal fragment, two oligonucleotides primers were synthesized and used to generate a cDNA fragment by the PCR method. The specificity of the PCR synthesized 220 bp fragment was verified by hybrid-arrest translation and sequencing. In situ hybridization performed with this cDNA fragment on Nereis diversicolor whole body defined two specific sites of recognition: a cluster of cells floating in the coelom and two types of muscles (perineural and oblique). Finally, the chronological expression of the MPII was postulated.

• 出版日期1993-2