Determination of paternal and maternal parentage in lodgepole pine seed: full versus partial pedigree reconstruction

作者：Funda Tomas; Liewlaksaneeyanawin Cherdsak; El Kassaby Yousry A^*

来源：Canadian Journal of Forest Research, 2014, 44(9): 1122-1127.

DOI：10.1139/cjfr-2014-0145

• lodgepole pine
• seed orchard
• partial and full pedigree reconstruction
• fertility variation
• mating system

Estimating seed orchards%26apos; maternal and paternal gametic contributions is of importance in assessing the genetic quality of seed crops. The advantage of full over partial pedigree reconstruction in investigating the mating dynamics in a lodgepole pine (Pinus contorta Dougl. ex. Loud. ssp. latifolia Engelm.) seed orchard population (N = 74) was demonstrated using nuclear and chloroplast microsatellite markers. We analyzed offspring of equivalent sample sizes representing full (bulk seed with unknown maternal and paternal parentage (n = 635)) and partial (11 maternal family arrays (n = 619)) pedigree reconstruction methods. Small differences in selfing rate, gene flow, and male reproductive success were observed between the two methods; however, the full pedigree reconstruction enabled simultaneous estimation of female-related fertility parameters (female reproductive success and effective number of maternal parents) that partial pedigree reconstruction could not provide. The use of bulk random sample of seed from orchards%26apos; crops is recommended when male and female fertility parameters, as well as selfing and contamination rates, are needed for seed orchards%26apos; seed crops genetic rating.

• 出版日期2014-9