Limiting Energy Dissipation Induces Glassy Kinetics in Single-Cell High-Precision Responses

作者：Das Jayajit^*

来源：Biophysical Journal, 2016, 110(5): 1180-1190.

DOI：10.1016/j.bpj.2016.01.022

Single cells often generate precise responses by involving dissipative out-of-thermodynamic -equilibrium processes in signaling networks. The available free energy to fuel these processes could become limited depending on the metabolic state of an individual cell. How does limiting dissipation affect the kinetics of high-precision responses in single cells? I address this question in the context of a kinetic proofreading scheme used in a simple model of early-time T cell signaling. Using exact analytical calculations and numerical simulations, I show that limiting dissipation qualitatively changes the kinetics in single cells marked by emergence of slow kinetics, large cell-to-cell variations of copy numbers, temporally correlated stochastic events (dynamic facilitation), and ergodicity breaking. Thus, constraints in energy dissipation, in addition to negatively affecting ligand discrimination in T cells, can create a fundamental difficulty in determining single-cell kinetics from cell-population results.

• 出版日期2016-3-8