科研之友
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摘要
A simple, highly sensitive and label-free electrochemical impedance spectroscopy (EIS) immunosensor was developed using Nafion and gold nanoparticles (nano-Au/Nafion) composites for the determination of 1-pyrenebutyric acid (PBA). Under the optimal conditions, the amount of immobilized antibody was significantly improved on the nano-Au/Nafion electrode due to the synergistic effect and biocompatibility of Nafion film and gold nanoparticles composites. The results showed that the sensitivity and stability of nano-Au/Nafion composite electrode for PBA detection were much better than those of nano-Au modified glassy carbon electrode (nano-Au/GCE). The plot of increased electron transfer resistances (Rets) against the logarithm of PBA concentration is linear over the range from 0.1 to 150 ng center dot mL(-1) with the detection limit of 0.03 ng center dot mL(-1). The selectivity and accuracy of the proposed EIS immunosensor were evaluated with satisfactory results.
