Higher interleukin-18 and mannose-binding lectin are present in uterine lumen of patients with unexplained infertility

作者：Oger P; Bulla R; Tedesco F; Portier A; Dubanchet S; Bailly M; Wainer R; Chaouat G; Ledee N^*

来源：Reproductive BioMedicine Online, 2009, 19(4): 591-598.

DOI：10.1016/j.rbmo.2009.05.011

• embryo transfer
• endoluminal uterine fluid
• interleukin-18
• IVF
• mannose-binding lectin
• uterine flushing

The uterine luminal environment was explored with regard to interleukin-18 (IL-18) and mannose-binding lectin (MBL) and the possibility that the procedure Of flushing the uterine cavity would optimize the physiological initial pseudo-inflammatory uterine reaction. Uterine flushings were performed among 175 IVF/intracytoplasmic sperm injection (ICSI) patients at the time of oocyte retrieval and the cycles were compared with a control group matched for age, number of previous attempts and type of assisted reproductive procedure (IVF or ICSI) in which no flushing were performed (n = 175). Samples collected were divided into two groups according to the presence/absence of endometrial cells in samples. IL-18 and MBL expressions were explored by enzyme-linked immunosorbent assay. Implantation rates were significantly higher in those patients who underwent the uterine flushing compared with controls (P = 0.04). Luminal concentrations of IL-18 and MBL were higher if endometrial cells were present in flushings. Suggesting endometrial origin of the secretion. Both concentrations of MBL and IL-18 were higher in patients with unexplained infertility compared with patients involved in IVF/ICSI for male or tubal infertility (P = 0.005 and 0.02, respectively). The exploration of the endoluminal environment before oocyte retrieval may enhance pregnancy rates and show distinct features in patients with unexplained infertility.

• 出版日期2009-10