Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size

作者：Perry Rotem Ben Tov; Rishal Ida; Doron Mandel Ella; Kalinski Ashley L; Medzihradszky Katalin F; Terenzio Marco; Alber Stefanie; Koley Sandip; Lin Albina; Rozenbaum Meir; Yudin Dmitry; Sahoo Pabitra K; Gomes Cynthia; Shinder Vera; Geraisy Wasim; Huebner Eric A; Woolf Clifford J; Yaron Avraham; Burlingame Alma L; Twiss Jeffery L; Fainzilber Mike

来源：Cell Reports, 2016, 16(6): 1664-1676.

DOI：10.1016/j.celrep.2016.07.005

How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin beta 1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin beta 1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin beta 1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells.

• 出版日期2016-8-9