Mapping, expression and regulation of the TR alpha gene in porcine adipose tissue

作者：Cai, Z. -W.; Sheng, Y. -F.; Zhang, L. -F.; Wang, Y.; Jiang, X. -L.; Lv, Z. -Z.; Xu, N. -Y.^*

来源：Genetics and Molecular Research, 2011, 10(3): 1320-1330.

DOI：10.4238/vol10-3gmr1113

• Thyroid hormone
• TR alpha
• Adipose tissue
• Fat deposition

Thyroid hormone receptors (TR) are members of the nuclear receptor superfamily. There are at least two TR isoforms, TR alpha and TR beta. The TR alpha isoform plays a critical role in mediating the action of thyroid hormone in adipose tissue. We mapped the porcine TR alpha gene to chromosome 12 p11-p13, by using the ImpRH panel. We examined tissue-localization of TR alpha and determined expression patterns of TR alpha in porcine adipose tissue with quantitative real-time PCR. TR alpha was expressed in all tissues, including heart, liver, spleen, stomach, pancreas, brain, small intestine, skeletal muscle, and subcutaneous adipose tissue. In the adipose tissue, the expression of TR alpha decreased postnatally. Compared to Yorkshire pigs, Jinhua pigs had significantly lower expression levels of TR alpha gene in the subcutaneous fat tissue. The expression levels of beta 2-AR, HSL and ATGL were also significantly lower in Jinhua pigs than in Yorkshire pigs. However, no significant differences in PPAR gamma and SREBP-1C expression levels were found between Jinhua and Yorkshire pigs. Incubation of porcine adipose tissue explants with high doses of isoproterenol (100 and 1000 nM) significantly increased the expression levels of TR alpha. We conclude that there is considerable evidence that TR alpha plays an important role in fat deposition in porcine adipose tissue.

• 出版日期2011
• 单位浙江大学