Antithrombotic Phenolics from the Stems of Parthenocissus tricuspidata Possess Anti-inflammatory Effect

作者：Phi Hung Nguyen; Zhao Bing Tian; Lee Jeong Hyung; Kim Young Ho; Min Byung Sun; Woo Mi Hee^*

来源：BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2014, 35(6): 1763-1768.

DOI：10.5012/bkcs.2014.35.6.1763

• Parthenocissus tricuspidata
• Anticoagulants
• Thrombin inhibitors
• NO production inhibitors
• Anti-inflammatory activity

In the course of our program to search for antithrombotic and anti-inflammatory agents from plants, twelve phenolics (1-12) were isolated from the stems of Parthenocissus tricuspidata. Their structures were elucidated on the basis of spectroscopic (1D and 2D NMR, and MS) data analyses, and comparison with published data. At the concentration of 100 mu g/mL, compounds 2, 4, 6 and 10 possessed potential effects on anti-blood coagulation, with inhibitory percentage of 216, 174, 148 and 225%, respectively; while aspirin used as positive control showed 181% inhibition at the same concentration. Furthermore, the anti-inflammatory activity of isolated compounds (1-12) was investigated on lipopolysaccharide (LPS)-induced murine macrophage cells (RAW264.7). Compounds 2,4 and 6 also potential inhibited the production of nitric oxide, with IC50 values of 11.9 +/- 0.3, 2.9 +/- 0.2 and 29.0 +/- 0.6 mu M, respectively. Celastrol, the positive control used, gave an IC50 value of 1.0 +/- 0.1 mu M.

• 出版日期2014-6-20