Antioxidant Effect of Human Selenium-containing Single-chain Fv in Rat Cardiac Myocytes

作者：Huo Rui; Shi Yi; Xu Jun Jie; Yan Fei; Lue Shao wu; Su Jia ming; Duan Yu jing; Fan Jia; Ning Bo; Cong Deng li; Yan Gang lin^*; Luo Gui min; Wei Jing yan

来源：Chemical Research in Chinese Universities, 2009, 25(2): 216-219.

• Human single-chain Fv
• Selenium
• Glutathione peroxidase
• H(2)O(2)
• Cardiac myocyte

Reactive oxygen species (ROS) plays a key role in human heart diseases. Glutathione peroxidase (GPX) functions as an antioxidant as it catalyzes the reduction of hydroperoxide. In order to investigate the antioxidant effect of human selenium-containing single-chain Fv(Se-scFv-B3), a new mimic of GPX, a model system of hydrogen peroxide (H(2)O(2))-induced rat cardiac myocyte damage was established. The cardiac myocyte damage was characterized in terms of cell viability, lipid peroxidation, cell membrane integrity, and intracellular H(2)O(2) level. The Se-scFv-B3 significantly reduced H(2)O(2)-induced cell damage as shown by the increase of cell viability, the decline of malondialdehyde (MDA) production, lactate dehydrogenase (LDH) release, and intracellular H(2)O(2) level. So Se-scFv-B3 may have a great potential in the treatment of human heart diseases induced by ROS.

• 出版日期2009-3
• 单位吉林大学