Antihyperalgesic and antidepressive actions of ( R)-(+)-limonene, alpha-phellandrene, and essential oil from Schinus terebinthifolius fruits in a neuropathic pain model

作者：Piccinelli Ana Claudia; Santos Joyce Alencar; Konkiewitz Elisabete Castelon; Oesterreich Silvia Aparecida; Nazari Formagio Anelise Samara; Croda Julio; Ziff Edward Benjamim; Leite Kassuya Candida Aparecida

来源：Nutritional Neuroscience, 2015, 18(5): 217-224.

DOI：10.1179/1476830514Y.0000000119

• Schinus terebinthifolius fruits
• Rat
• Depression
• Neuropathic pain
• Limonene
• Phellandrene

Objectives: Previous studies have shown that essential oil containing (R)-(+)-limonene and alpha-phellandrene, extracted from fruits of Schinus terebinthifolius Raddi, exhibit anti-inflammatory activity. This work aimed to verify the antihyperalgesic and antidepressive actions of (R)-(+)-limonene, a-phellandrene, and essential oil from S. terebinthifolius fruits in spared nerve injury (SNI) model of neuropathic pain in rats.
Methods: In the present work, essential oil from fruits of S. terebinthifolius, as well as the pure (R)(+)- limonene and a-phellandrene compounds, were assayed for their effects on SNI-induced mechanical and cold hyperalgesia, and depressive-like behavior (immobility in forced swim test) in rats. The locomotor activity was evaluated in open-field test.
Results: Oral administration for up to 15 days of essential oil of S. terebinthifolius (100 mg/kg), (R)-(+)-limonene (10 mg/kg), a-phellandrene (10 mg/kg), and also subcutaneous 10 mg/kg dose of ketamine (positive control) significantly inhibited SNI-induced mechanical hyperalgesia and increased immobility in the forced swim test. On the 15th day of oral treatment, a-phellandrene, but neither the essential oil from S. terebinthifolius nor (R)-(+)-limonene, prevented the SNI-induced increase in sensitivity to a cold stimulus. The oral treatment with essential oil (100 mg/kg) or with compounds (10 mg/kg) did not interfere on locomotor activity.
Discussion: Together, the results of the present work show that essential oil of S. terebinthifolius and compounds present in this oil, including (R)-(+)-limonene and a-phellandrene, exhibit antihyperalgesic effects against mechanical hyperalgesia, and are antidepressive, while only a-phellandrene inhibited cold hyperalgesia in SNI rats.

• 出版日期2015-7