Ubiquitylation-dependent downregulation of Nck regulates its functional activity

作者：Joseph Noah; Reicher Barak; David Ahuvit; Matalon Omri; Barda Saad Mira^*

来源：FEBS LETTERS, 2014, 588(21): 3808-3815.

DOI：10.1016/j.febslet.2014.08.033

• Ubiquitylation
• Nck
• Actin machinery
• T-cell signaling

The Nck adapter protein is involved in key cellular functions, such as actin polymerization and reorganization, serving as a molecular bridge between the surface complex essential for foreign antigen recognition, the T-cell antigen receptor (TCR), and the actin machinery. However, the mechanisms regulating Nck expression and functions are unknown. In this study, we revealed Nck negative regulation and demonstrated that Nck is ubiquitylated following cellular activation. We identified the molecular determinants and mediators involved in this process. Our data suggest that Nck ubiquitylation might serve as a mechanism controlling Nck-mediated effector functions during cellular activation. %26lt;br%26gt;Structured summary of protein interactions:

• 出版日期2014-11-3