Description of a novel marine bacterium, Vibrio hyugaensis sp nov., based on genomic and phenotypic characterization

作者：Urbanczyk Yoshiko; Ogura Yoshitoshi; Hayashi Tetsuya; Urbanczyk Henryk^*

来源：Systematic and Applied Microbiology, 2015, 38(5): 300-304.

DOI：10.1016/j.syapm.2015.04.001

• Vibrio hyugaensis sp nov.
• Vibrionaceae
• Vibrio
• Harveyi clade
• Genomic taxonomy

Three luminous bacteria strains have been isolated from seawater samples collected in the coastal regions of the Miyazaki prefecture in Japan. Analysis of the 16S rRNA gene sequences identified the three strains as members of the genus Vibrio (Vibrionaceae, Gammaproteobacteria), closely related to bacteria in the so-called 'Harveyi clade.' The genomes of the three strains were estimated to be between 5.49 Mbp and 5.95 Mbp, with average G + C of 43.91%. The genome sequence data was used to estimate relatedness of the three strains to related Vibrio bacteria, including estimation of frequency of recombination events, calculation of average nucleotide identity (ANI), and a phylogenetic analysis based on concatenated alignment of nucleotide sequences of 135 protein coding genes. Results of these analyses in all cases showed the three strains forming a group clearly separate from previously described Vibrio species. A phenotypic analysis revealed that the three strains have character similar to Vibrio bacteria in the 'Harveyi clade', but can be differentiated from previously described species by testing for hydrolysis of esculin. Based on results of genomic, phylogenetic and phenotypic analyses presented in this study, it can be concluded that the three strains represent a novel species, for which the name Vibrio hyugaensis sp. nov. is proposed. The type strain is 090810a(T) (=LMG 28466(T) = NBRC 110633(T)).

• 出版日期2015-7