科研之友
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摘要
The metabolic structure and successional events of microbial communities in pentachlorophenol (PCP) contaminated soils were evaluated and compared in three biological treatments. These were: biostimulation of indigenous populations with nutrients in soils contaminated with 75,150, and 250 mg/kg of PCP (dry weight), a set of reactors initially seeded with an active PCP-degrading bacterial consortium, and soil bioaugmented with known PCP-degrading strains of Sphingomonas chlorophenolica (ATCC 53874 and ATCC 33790). Soil with no addition of PCP was used as a native community control. Although higher PCP concentration enhanced toxicity after 35 days of biostimulation resulted in 92-95% depletion of PCP. On the contrary, when compared to non-inoculated systems, biciaugmentation with S. chlorophenolica produced an accelerated PCP depletion at initial concentrations of 150 and 250 mg/kg. Community-level Biolog GN and GP microtitre plates were used to examine the metabolic structure of soil degrading communities. Principal component analysis of substrate utilization patterns revealed changes in the biological structure of all PCP amended systems when compared with PCP-free controls. All inoculated systems with S. chlorophenolica converged with patterns observed in PCP-free soil communities while the other biological treatments maintained a distinctive profile. The convergence of PCP exposed community with the original soil community structure suggests that chemical and ecological restoration occurred.
- 出版日期2005
